Dual Luciferase Reporter Assay System Promega
Dual Luciferase Reporter Assay System
Dual luciferase reporter assay system promega. For others we wont set them unless you accept them. Ultra-sensitive detection of firefly and NanoLuc luciferase activities in a single sample. To sensitively measure both Fluc and NanoLuc Nluc in the same sample we have developed the Nano-Glo Dual-Luciferase Reporter NanoDLR Assay System a homogeneous lytic assay performed in an add-read-add-read format in which the Fluc signal is.
Format of the Dual-Luciferase Reporter 1000 Assay in 96-Well Plates The DLR 1000 Assay may be performed directly in 96-well plates immediately following lysate preparation without. Ad Reagents for Colorimetric and Fluorescent Assays. Ad Reagents for Colorimetric and Fluorescent Assays.
The convenient add-mix-measure protocol generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. Some of these cookies are essential for our website to work. See promotional offers below.
The firefly luciferase activities were corrected by the corresponding renilla luciferase activities. For dual-luciferase assay using B and epithelial cells Fig. Reporter plasmids and siRNAs were introduced into PC9 cells as in c.
One from firefly Photinus pyralis and the second from Renilla Renilla reniformis. Total elapsed time for the DLRŽ Assay 30 seconds Promega Corporation. Revised 806 Part TM040 AF9TM040 0806TM040 tm0400806qxp 8112006 249 PM Page 1.
The widetype and mutated promoter sequence of SLC7A11 were provided in supplemental materials S1. Dual-Luciferase Reporter Assay System. Dual-Glo Luciferase Assay System.
In the DLR Assay the activities of firefly and Renilla luciferases are measured sequentially from a single sample. In the DLR Assay the activities of firefly Photinus pyralis and Renilla Renilla reniformis or sea pansy luciferases are measured sequentially from a single sampleThe firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II LAR II to generate.
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The widetype and mutated promoter sequence of SLC7A11 were provided in supplemental materials S1.
Dual-Luciferase Reporter Assay System INSTRUCTIONS FOR USE OF PRODUCTS E1910 AND E1960. Incubate at 2025C for 10 minutes2 hours. Reporter plasmids and siRNAs were introduced into PC9 cells as in c. From classic Dual-Luciferase Assays to state-of-the-art NanoLuc Luciferase we offer a range of detection options with unrivaled sensitivity and versatility. In the DLR Assay the activities of firefly Photinus pyralis and Renilla Renilla reniformis or sea pansy luciferases are measured sequentially from a single sampleThe firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II LAR II to generate. Promega Dual-Luciferase Reporter DLR Assay Systems. In the DLR Assay the activities of firefly Photinus pyralis and Renilla Renilla reniformis also known as sea pansy luciferases are. After 24h incubation Dual-luciferase assay was carried out as in Fig 1b n 6. The Dual-Glo Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample.
The Dual-Luciferase Reporter DLR Assay Systemac provides an efficient means of performing dual-reporter assays. The firefly luciferase activities were corrected by the corresponding renilla luciferase activities. For dual-luciferase assay using B and epithelial cells Fig. Detect reporter gene activity with luciferase-based assays just right for your application. From classic Dual-Luciferase Assays to state-of-the-art NanoLuc Luciferase we offer a range of detection options with unrivaled sensitivity and versatility. Reporter plasmids and siRNAs were introduced into PC9 cells as in c. We use cookies and similar technologies to make our website work run analytics improve our website and show you personalized content and advertising.
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